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　　Tridimensional Visualization and Analysis of Early Human Development

　　早期人类胚胎发育的凤凰网站三维可视化和分析

　　Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France

　　?We used 3DISCO/iDISCO+ clearing methods to start building a 3D human cell atlas

　　?我们使用3DISCO/ iDISCO+ 的显影方法开始构建 三维的人体细胞图谱。

　　?A collection of high-resolution images of whole human embryos and fetal organs

　　?高分辨率图谱的集合 包括了凤凰娱乐网站人类全胚胎和胎儿的各器官。

　　?An interactive online resource and 3D digital database of human development

　　?可以使用交互式在线资源和 三维数字数据库检索 人类发育的进程。

　　?Unknown features of human development revealed in cleared and immunostained embryos

　　?人体发育的未知功能 相性隐藏在免疫显影的胚胎造影中。

　　Generating a precise cellular and molecular cartography of the human embryo is essential to our understanding of the mechanisms of organogenesis in normal and pathological conditions.凤凰平台

　　一个精确的人类胚胎细胞和分子制图图谱，对于我们对正常或病理条件下器官形成的机制的理解至关重要的。

　　Here, we have combined whole-mount immunostaining, 3DISCO clearing, and light-sheet imaging to start building a 3D cellular map of the human development during the first trimester of gestation.

　　本研究中，我们结合包埋免疫染色，三维DISCO显影，片成扫描三维重构 怀孕的前三个月人体发育胚胎的细胞图谱。

　　We provide high-resolution 3D images of the developing peripheral nervous, muscular, vascular, cardiopulmonary, and urogenital systems.

　　我们提供高分辨率的三维图像，描叙神经、肌肉、血管、心肺,泌尿生殖系统的发育。

　　We found that the adult-like pattern of skin innervation is established before the end of the first trimester, showing important intra- and inter-individual variations in nerve branches.

　　我们发现胚胎在三个月结束前就形成了成人的皮肤神经支配模式建立了,显示重要的内部结构的神经分支的变化。

　　We also present evidence for a differential vascularization of the male and female genital tracts concomitant with sex determination.

　　我们还提供证据显示：微分血管化由男性和女性生殖道相伴与性别决定的相关性。

　　This work paves the way for a cellular and molecular reference atlas of human cells, which will be of paramount importance to understanding human development in health and disease.

　　这项工作为人类细胞的细胞和分子参考图集开创了新的里程碑,

　　这将是非常重要的,理解人体的发育生物学以及对人类健康和疾病的理解。

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